LINKAGE MAPPING OF CAROTENOID CLEAVAGE DIOXYGENASE-4 FAMILY IN LENTIL GENOME

Abstract

Plant carotenoid cleavage dioxygenases (CCDs) are a family of enzymes that catalyze the carotenoids oxidative cleavage and apocarotenoids and also play a crucial role in plant development and growth. CDD4 is a member of CDDs. It has two isoforms as CCD4a and CCD4b and they encode enzymes to catalyze the cleavage of carotenoids forming pigment compounds and aroma. In this study, CDD4 genes were mapped for the first time in the linkage group of lentil. CsCCD4af was located at 102.3 cM on linkage group 3 (LG3). CCD4-P-r1-1 and CCD4-P-r1-2 were located at 75.5 cM and 82.9 cM on LG3, respectively. CCD4-P-r1-3 was located at 151.6 cM on LG5. CsCCD4a/b-r was amplified but could not mapped due to its monomorphic band profile between parents. Location of these genes on the linkage map of lentil will help breeders improve strategies in order to generating new cultivars with higher carotenoid concentration.