EFFECTS OF LEAF AGE AND ENZYME DIGESTION PERIOD ON THE PROTOPLAST YIELD IN POTATO
Firdevs NIKSARLI , Zihin YILDIRIM
Abstract
Excised leaves from 5-7 week old greenhouse plants of two genotypes, Clone 6-7 and Granola, were preconditioned for 2 days in the dark at 4° C and 1 day in the dark in the conditioning medium for protoplast isolation. Samples were digested in enzyme solution for 8 and 16 hours. Then they were purifled by centrifugation in Babcock bottles. Washed protoplasts were counted with a hemocytometer and protoplast yield was calculated. It was found that protoplast yiled was affected by enzyme digestion period and leaf age as well as by genotype.
Keyword: greenhouse plants ,centrifugation ,Excised leaves ,
Effects of Different Water Stress Levels on Biomass Yield and Agronomic Traits of Switchgrass (Panicum virgatum L.) Varieties under Semi-Arid Conditio
Erdal GONULAL, Suleyman SOYLU, Mehmet SAHIN
Abstract
A field experiment was conducted in the Wielkopolska region at the Gorzyń Research Station, Poland (52.34°N,
15.54°E) in Central Europe. The study was conducted over a 3-year period (2017, 2018, 2019) as a two-factorial
design with four replications in the RCBD. The aim of the research was to determine the effect of the cultivar
(‘Bolero’, ‘Tytan’) and the inoculation (Nitragina–seeds inoculation, Nitroflora I–seeds inoculation, Nitroflora
II–soil inoculation, HiStick® Lupin–seeds inoculation) on plant development, seeds chemical composition and
yielding of narrow-leaved lupin. The weather conditions and experimental factors significantly influenced on
productivity of narrow-leaved lupin ‘Tytan’. Drought during the growing season reduced seeds and protein
yields. After inoculation of HiStck the seeds yield was significantly greater by 12.4% (p < 0.01) and the protein
yield after application of Nitroflora I or HiStick by 13.9% (p < 0.01) and 19.2% (p < 0.01), respectively.
Correlation coefficients showed strong relations between number of pods and seeds per plant in both cultivars
regardless of the inoculation variant, however the strongest relations in both cultivar were proved on HiStick
treatment.
Keyword: Biological nitrogen fixation, chemical composition, legumes, protein efficiency, yielding