Turkish Journal of Field Crops

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2. Beyler İş Hanı, No: 313 Kat: 3 Konak-İzmir

Agrobacterium-Mediated Transformation in Linseed (Linum usitatissimum L.)

Orhan Kurt

Abstract

An optimized regeneration protocol and a suitable transformation technique is a necessity in obtaining transgenic plants in a given plant species. This research summarizes a simple culture method to obtain rooted plants from stem pieces of linseed cultivar Antares within 3-4 months. Here, we also present our results on transformation experiments carried out with 7-day-old stem explants and Agrobacterium tumefaciens EHA105 strain. According to molecular examination 14 transgenic plants of 276 (5.1%) examined plants were obtained.

Keyword: Linseed, Agrobacterium tumefaciens, EHA105, genetic transformation ,

Effects of Different Water Stress Levels on Biomass Yield and Agronomic Traits of Switchgrass (Panicum virgatum L.) Varieties under Semi-Arid Conditio

Erdal GONULAL, Suleyman SOYLU, Mehmet SAHIN

Abstract

A field experiment was conducted in the Wielkopolska region at the Gorzyń Research Station, Poland (52.34°N, 15.54°E) in Central Europe. The study was conducted over a 3-year period (2017, 2018, 2019) as a two-factorial design with four replications in the RCBD. The aim of the research was to determine the effect of the cultivar (‘Bolero’, ‘Tytan’) and the inoculation (Nitragina–seeds inoculation, Nitroflora I–seeds inoculation, Nitroflora II–soil inoculation, HiStick® Lupin–seeds inoculation) on plant development, seeds chemical composition and yielding of narrow-leaved lupin. The weather conditions and experimental factors significantly influenced on productivity of narrow-leaved lupin ‘Tytan’. Drought during the growing season reduced seeds and protein yields. After inoculation of HiStck the seeds yield was significantly greater by 12.4% (p < 0.01) and the protein yield after application of Nitroflora I or HiStick by 13.9% (p < 0.01) and 19.2% (p < 0.01), respectively. Correlation coefficients showed strong relations between number of pods and seeds per plant in both cultivars regardless of the inoculation variant, however the strongest relations in both cultivar were proved on HiStick treatment.
Keyword: Biological nitrogen fixation, chemical composition, legumes, protein efficiency, yielding