Turkish Journal of Field Crops

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GENETIC DIVERSITY OF DIPLOID AND TETRAPLOID COTTONS DETERMINED BY SSR AND ISSR MARKERS

Adem BARDAK , Yuksel BOLEK

Abstract

Cotton as an annual crop is mainly grown for its fiber and oil in the seed. Determining genetic diversity in the germplasm is the first step of plant breeding. This study aimed to determine genetic diversity for diploid and tetraploid cotton genotypes grown in different parts of the world. SSR (Simple Sequence Repeats) and ISSR (Inter Simple Sequence Repeats) markers were used to determine genetic relationships among Gossypium species and genotypes. Using 39 SSR and 5 ISSR markers, 173 alleles (averaging 3.93 alleles per locus) were produced from 25 cotton genotypes. Out of 173 alleles, 155 (89.60%) were polymorphic among the genotypes and polymorphic information content (PIC) values were between 0.0040 and 0.9993 averaging 0.4396. Genetic diversity ranged from 0.04 to 0.58 among all the genotypes inspected. This ratio was 0.04-0.23 within G. hirsutum L. and 0.07-0.26 within G. barbadense L. species. Out of these two species, genetic diversity ranged from 0.23 to 0.57 among other diploid and tetraploid species. Genetic diversity was low within commercial cultivars that are also frequently used in breeding programs (0.08-0.20). It is advisable to use wild type cottons to increase present genetic diversity in germplasm pools to have a better chance for the selection of the desired traits.

Keyword: cotton, Gossypium, genetic diversity, molecular markers, diploid, tetraploid ,
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Effects of Different Water Stress Levels on Biomass Yield and Agronomic Traits of Switchgrass (Panicum virgatum L.) Varieties under Semi-Arid Conditio

Erdal GONULAL, Suleyman SOYLU, Mehmet SAHIN

Abstract

A field experiment was conducted in the Wielkopolska region at the Gorzyń Research Station, Poland (52.34°N, 15.54°E) in Central Europe. The study was conducted over a 3-year period (2017, 2018, 2019) as a two-factorial design with four replications in the RCBD. The aim of the research was to determine the effect of the cultivar (‘Bolero’, ‘Tytan’) and the inoculation (Nitragina–seeds inoculation, Nitroflora I–seeds inoculation, Nitroflora II–soil inoculation, HiStick® Lupin–seeds inoculation) on plant development, seeds chemical composition and yielding of narrow-leaved lupin. The weather conditions and experimental factors significantly influenced on productivity of narrow-leaved lupin ‘Tytan’. Drought during the growing season reduced seeds and protein yields. After inoculation of HiStck the seeds yield was significantly greater by 12.4% (p < 0.01) and the protein yield after application of Nitroflora I or HiStick by 13.9% (p < 0.01) and 19.2% (p < 0.01), respectively. Correlation coefficients showed strong relations between number of pods and seeds per plant in both cultivars regardless of the inoculation variant, however the strongest relations in both cultivar were proved on HiStick treatment.
Keyword: Biological nitrogen fixation, chemical composition, legumes, protein efficiency, yielding
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