Advanced pure lines, which are in different genetic and vegetative characteristics determined in a breeding program initiated in 2013, were included to the yield tests in the region before registration. Chemical and morphological variability and stability of 13 advanced pure tobacco lines and 2 standard varieties were determined in two different locations. The experimental layout was random blocks with three replications and the experiment was carried out in 2016 and 2017. Plant height values ranged from 85.25 to 156.71 cm, the number of leaves from 22.43 to 41.71 leaf plant-1 , leaf width ranged from 9.35 to 11.52 cm, leaf length from 17.36 to 21.89 cm, yield from 1371.9 to 1756.7 kg ha-1 , quality grade index from 67.33 to 81.58%, nicotine content from 1.02 to 1.98% and glucose content from 3.33 to 9.33%. The lines 16, 21, 26 and 45 were identified as strong variety candidates suitable for the region based on all the parameters and the statistical conditions examined. The use of biplots that the environments were distributed on axes, enabled to make location-based variety recommendations. The biplot graph based on environment and genotype interaction was used in the recommedations of tobacco variety for each location.