Drought is one of the most critical environmental stressors affecting agricultural productivity around the world and it considerably results in yield. Symbiosis interaction between plants and soil micro-organisms are considered to enhance plant tolerance in a/biotic conditions. In this study, the impact of Glomus mosseae, Pseudomonas fluorescens strain 169 and both of them under imposed water stress (flowering and pod filling stages) of mung bean (Vigna radiata (L.) Wilczek) was evaluated in two farm experiments during 2016 and 2017. Eco-physiological parameters have been recorded which showed that drought stress reduced the number of leaves, root colonization and seeds yield of mung bean. Mixed inoculation of G.mosseae and P.fluorescens 169 was more effective in alleviation the harm effects of drought stress. Enzymes assay suggested that co-inoculation of G.mosseae and P.fluorescens 169 was more effective to increase antioxidative defense system like catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GR) activities. H2O2 contents were increased by water stress both in cutting irrigation at flowering and pod filling stages. In conclusion, plants inoculated with combination of G.mosseae and P.fluorescens 169 had less oxidative damage over control plants.