Sunflower (Helianthus annuus L) has gained importance in the world as a significant oilseed for vegetable oil. The reactions of the same sunflower genotypes to varying ecologies are different, and yield and yield characteristics may vary depending on this. In this study, we were interested in understanding the differential sensitivity of specific sunflower genotypes to different agro-ecological environments in Turkey. A 2-year (2015–2016) study was conducted on a clay-loam soil in a low and high altitude environment to (i) evaluate the genotype × location × year interaction (G×L×Y) for sunflower genotypes, (ii) predict yield components performance and identify high stable sunflower genotypes, and (iii) make genotype-specific management and high performing genotype recommendations within and across agro-ecological regions. The highest yield of seed and oil yield was obtained from the Coral (G1) genotypes, averaging 3558.5 and 1535.6 kg ha-1 , respectively in Erzurum and from the 64LC108 (G4) genotypes averaging 2905.7 and 1267.4 kg ha-1 , respectively in Samsun ecological conditions. As a result, the determination of the sunflower genotypes suitable for the region will provide the opportunity to obtain high-yield and high quality products because the soil, environment and climatic factors of the region in which they are grown are very useful in determining seed yield and oil content.