Turkish Journal of Field Crops

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GENETIC DIVERSITY OF CROCUS SATIVUS AND ITS CLOSE RELATIVE SPECIES ANALYZED BY iPBS-RETROTRANSPOSONS

Aysun GEDIK , Duygu ATES , Semih ERDOGMUS , Gonul COMERTPAY , Muhammed Bahattin TANYOLAC , Hakan OZKAN

Abstract

Saffron (Crocus L.) is a member of Crocoideae, the biggest of four subfamilies in the Iridaceae family. It has 2n = 3x = 24 chromosomes and is triploid; thus, it is sterile. In previous research, different molecular DNA markers were used but molecular characterization and genetic diversity of this complex genus have not yet been clarified. Therefore, current study aimed to determine the molecular characterization of saffron and its close relative species using inter-primer binding site (iPBS)-retrotransposon markers. Eighty-three iPBSretrotransposon primers were used in 28 C. sativus genotypes and 17 close relative species of saffron to identify their genetic diversity. Sixteen polymorphic iPBS-retrotransposon primers generated a total of 401 polymorphic scorable bands. The mean PIC value, Nei’s genetic diversity and Shannon’s information index (I) were calculated as 0.85, 0.16 and 0.29, respectively. The results of the Unweighted Pair Group Method with Arithmetic mean UPGMA dendrogram and Principal Coordinates Analysis PCoA analysis indicated a spatial representation of the relative genetic distances among 28 saffron samples and the 17 close relative species were categorized under two distinct groups. Saffron genotypes showed very limited genetic variation and according to the iPBS-retrotransposon data, its close relatives were C. cartwrightianus and C. pallasii subsp. pallasii.

Keyword: Crocus sativus, genetic diversity, inter-primer binding site retrotransposons, saffron ,
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Effects of Different Water Stress Levels on Biomass Yield and Agronomic Traits of Switchgrass (Panicum virgatum L.) Varieties under Semi-Arid Conditio

Erdal GONULAL, Suleyman SOYLU, Mehmet SAHIN

Abstract

A field experiment was conducted in the Wielkopolska region at the Gorzyń Research Station, Poland (52.34°N, 15.54°E) in Central Europe. The study was conducted over a 3-year period (2017, 2018, 2019) as a two-factorial design with four replications in the RCBD. The aim of the research was to determine the effect of the cultivar (‘Bolero’, ‘Tytan’) and the inoculation (Nitragina–seeds inoculation, Nitroflora I–seeds inoculation, Nitroflora II–soil inoculation, HiStick® Lupin–seeds inoculation) on plant development, seeds chemical composition and yielding of narrow-leaved lupin. The weather conditions and experimental factors significantly influenced on productivity of narrow-leaved lupin ‘Tytan’. Drought during the growing season reduced seeds and protein yields. After inoculation of HiStck the seeds yield was significantly greater by 12.4% (p < 0.01) and the protein yield after application of Nitroflora I or HiStick by 13.9% (p < 0.01) and 19.2% (p < 0.01), respectively. Correlation coefficients showed strong relations between number of pods and seeds per plant in both cultivars regardless of the inoculation variant, however the strongest relations in both cultivar were proved on HiStick treatment.
Keyword: Biological nitrogen fixation, chemical composition, legumes, protein efficiency, yielding
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