The effects of chicken manure and different bacteria applications on yield and yield components of beans were investigated in present study. The field experiment was conducted during the 2019 and 2020 at the experimental area of the Faculty of Agriculture, Eskisehir Osmangazi University, Eskisehir, Turkey. Experiment laid out as split plot based on randomized complete block design with three replications. Chicken manure (chicken manure + and chicken manure -) were main plots and bacteria applications (control; traditional; rhizobia; phosphorus dissolving bacteria; traditional + rhizobia; traditional + phosphorus dissolving bacteria; rhizobia + phosphorus dissolving bacteria; traditional + rhizobia + phosphorus dissolving bacteria) were sub-plots. All of the investigated characters were higher in second year than first year except for harvest index and hundred seed weight due to especially June precipitation in second year. Grain yield increased from 1233 kg/ha to 3111 kg/ha in the second year of the experiment. Application of chicken manure positively affected important yield components, chlorophyll content and yield. Grain yield increased by approximately 5% with the application of chicken manure. In general, the application of bacteria alone or together has positively affected yield and yield components. The highest yield was obtained from traditional+rhizobia+phosphorus dissolving bacteria application. Grain yield in terms of bacterial applications varied between 1915 and 2488 kg/ha. Consequently, it was determined that the efficiency of bacteria increased with the application of chicken manure and positive results were obtained in yield and yield components of beans.