Lathyrus sativus L. (grass pea) is an annual legume crop commonly cultivated in marginal areas and has been used as food and feeding. This study was conducted to characterize 15 superior grass pea genotypes grown in two locations (Antalya and Isparta) with respect to fatty acids, oil content and oil yield (Seed yield (g plant-1 ) × Oil content (%)). Grass pea seeds were sown in a randomized complete blocks design and an augmented experimental design in Antalya and Isparta, respectively. In the first step of study, the seeds were harvested on 25 May 2021 and 15 June 2021 in Antalya and Isparta respectively. Linoleic acid was the dominant fatty acid present in all grass pea genotypes, with its contents ranging from 39.38% (GP213) to 42.61% (GP150). Lauric, tridecanoic, pentadecanoic, palmitic and erucic acid were found at trace levels; meanwhile, oleic acid was determined as the second excess fatty acid in all genotypes, ranging from 19.12 to 21.41%. The amounts of saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids were calculated to be in the ranges of 23.82-28.57%, 19.63-22.36% and 51.06-54.43%, respectively. The oil ratios and oil yields of the genotypes varied between 0.59 and 0.80% and between 0.01 and 0.24 g plant-1 . The significance t-test for mean values indicated that there were no significant differences between the locations for all fatty acid features and oil traits. These presented data showed these collection presents new superior lines with respect to oil traits.