RESEARCH ON SINGLE PLANT CHARACTERISTICS IN MULTI-SPIKE TYPE WHEAT IN SOUTHWEST CHINA BASED ON THE STEREOSCOPIC ANATOMICAL OBSERVATION AND THE ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA) RUNNING TITLE: SINGLE PLANT CHARACTERISTICS

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HAO Yan-Ling, ZHANG Zi-Jin, ZHANG Huai-Yu, REN Zheng- Long

Abstract

To research on the characteristics of tillers and spike formation per plant and their relationship with the composition factors of yield in multi-spike type wheat in the special ecological environment in southwest China. 3 spike types of wheat were selected to use as experimental materials, with the methods of investigation in field, the stereoscopic anatomical observation and the enzyme linked immunosorbent assay(ELISA).The results showed that comparing with the other 2 spike-types wheat, the tillers per plant have the least time (7.41 days) for appearing and the least interval time(2.66 days)and the primordia difference value(1.10), the content differences in GAs, IAA, ZR and ABA (0.59, 1.01, 0.129, 1.01 ng.g-1FW) are the minimum on average for adjacent tiller. The ratios of IAA/ABA (0.65), ZR/ABA (0.08) in secondary tillers are the highestbut the IAA/(ZR+ZT) (1.06) is the lowest. Average difference values per panicle traits for adjacent tillers on spike grain number (1.69), grain weight of single ear panicle (2.31mg), infertility spikelet (0.87) and fertility spikelet (1.61) are the minimum, but earring percentage per plant (87.50%) and grain weight per plant (23.75g) are the highest after mature. There are the more coordination and synchronism for all tillers in the multi-spike type wheat, which is the most beneficial to build a reasonable yield component and breed a breakthrough variety with high yield in this area compared with the other 2 spike-types of wheat.

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