STUDY ON DIFFERENCE OF FUNGAL COMMUNITY STRUCTURE IN EARLY JAPONICA PADDY WITH DIFFERENT MOISTURE CONTENTS

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Li Xinwei, Gao Shucheng, Cao Yi,Hao Liqun, Zhao Xu, Gao Xianglan, Li Jia

Abstract

With newly harvested early Japonica paddy in northeast China with different moisture contents as subjects, the differences in fungal community structure and diversity were analyzed, the original state of fungal community in paddy with different moisture contents before warehousing was investigated, and unculturable fungal communities were explored comprehensively using Illumina MiSeq high-throughput sequencing, so as to provide a basis for the exploration of the change rule of fungal community and the establishment of anti-mildew system during paddy storage. The results showed that according to 97% sequence similarity, the fungal communities in the paddy samples with two moisture contents belonged to 2 phyla, 26 classes, 51 orders, 85 families, 112 genera and 188 species. At the phylum level, Ascomycota and Basidiomycota were the dominant phyla of the fungal communities in paddy with two moisture contents. In high-moisture paddy, the abundance of Ascomycota and Basidiomycota was 56.96% and 7.41%, respectively. In low-moisture paddy, the abundance of Ascomycota and Basidiomycota was 51.37% and 2.898%, respectively. At the genus level, the 20 genera with high abundance in the paddy with two moisture contents included Cryptococcus, Microdochium, Fusarium, Rhodosporidiobolus, Bulleromyce, Sarocladium, Symmetrospor, Dioszegia, Sporobolomyces, Kondoa, Setophoma, Coniothyrium, Didymella, Filobasidium, Moesziomyces, Curvularia, Mycosphaerella, Papiliotrema, Neosetophoma and Alternaria. Cryptococcus and Papiliotrema contents were high in high-moisture paddy, but low in low-moisture paddy. Filobasidium, Sarocladium and Mycosphaerella were specific genera in high-moisture paddy, but did not exist in low-moisture paddy. Through Ven analysis and PCA, it was found that although there were a large number of the same species in paddy with different moisture contents, species abundance was different, and the species scale and quantity of fungi in high-moisture paddy were larger than those in low-moisture paddy.

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